Artifactual Sulfation of Silver-stained Proteins

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

Proteins from silver-stained gels can be digested enzymatically and the resulting peptides analyzed and sequenced by mass spectrometry. Standard proteins yield the same peptide maps when extracted from Coomassieand silver-stained gels, as judged by electrospray and MALDI mass spectrometry. The low nanogram range can be reached by the protocols described here, and the method is robust. A silver-...

Quantitative computerized analysis of silver-stained and Coomassie Blue-stained two-dimensional protein maps.

Using the two-dimensional gel electrophoresis and two systems for computer analysis of the resulting maps, we obtained a good quantitative reproducibility of individual spots of silver-stained and Coomassie Blue-stained gels from mouse fetus liver samples. The GelScan-XL system was established for the evaluation of silver-stained gels. The Gel-Image software has shown to be capable of the analy...

The Nerve Elements in Silver-stained Preparations of Cordylophora.

SYNOPSIS. The nervous sjstem in the hydranth of Cordylophora was stained by a modified Peters' silver method. Nerve elements are found onl) in the ectoderm, where the) form a dense plexus, oriented mainly longitudinally. The majoriu of the nerve cells, whether lying deep in the ectoderm or near the surface, carry a short hair, presumably sensory. These findings are compared to earlier reports o...

Norethindrone scatters silver-stained nucleolar organizer regions of Ishikawa cells.

We investigated the effects of sex steroids on silver-stained nucleolar organizer regions (Ag-NORs) and DNA/RNA kinetics in Ishikawa cells. Norethindrone and its isomer norethynodrel exclusively caused Ag-NORs to scatter in the nuclear matrix, from the nucleolus. No such effect occurred with the other sex steroids tested, including progestational, androgenic, and estrogenic compounds. Nuclear a...

Western blot of stained proteins from dried polyacrylamide gels.